1PMM
Crystal structure of Escherichia coli GadB (low pH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.780, 91.406, 93.686 |
| Unit cell angles | 76.80, 77.10, 78.24 |
Refinement procedure
| Resolution | 25.000 * - 2.000 |
| Rwork | 0.182 |
| R-free | 0.21300 |
| Structure solution method | MAD |
| Starting model (for MR) | NULL |
| RMSD bond length | 0.005 |
| RMSD bond angle | 21.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.162 |
| Number of reflections | 178481 | |
| <I/σ(I)> | 9.3 | 6.3 |
| Completeness [%] | 95.8 | 94.6 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 293 | sodium formate, sodium acetate, PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 100 (mM) | pH4.6 |
| 3 | 1 | reservoir | sodium acetate | 135 (mM) | pH4.6 |
| 4 | 1 | reservoir | sodium formate | 700 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 13 (%) |
