1PMI

Candida Albicans Phosphomannose Isomerase

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Detector technology	IMAGE PLATE
Collection date	1994-03-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	124.830, 52.920, 85.730
Unit cell angles	90.00, 127.54, 90.00

Refinement procedure

Resolution	7.000 - 1.700
R-factor	0.184 *
Rwork	0.184
RMSD bond length	0.022
RMSD bond angle	0.027
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.052
Number of reflections	45325
Completeness [%]	85.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	PEG8000	20 (%(w/v))
3	1	reservoir	calcium acetate	200 (mM)
4	1	reservoir	MES	25 (mM)