1PMD
PENICILLIN-BINDING PROTEIN 2X (PBP-2X)
Experimental procedure
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 161.250, 161.250, 170.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 3.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.21100 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.062 * |
| Number of reflections | 16436 |
| Completeness [%] | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * | 15 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | PEG10000 | 4 (%) | |
| 3 | 1 | drop | acetate | 50 (mM) | |
| 4 | 1 | reservoir | peg10000 | 8 (%) | |
| 5 | 1 | reservoir | acetate | 100 (mM) |
