1PM3
MTH1859
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-03 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 154.700, 154.700, 68.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.150 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.29300 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.100 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 | 0.463 |
| Number of reflections | 18202 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.8 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | VAPOR DIFFUSION, HANGING DROP, pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.0 |
| 3 | 1 | reservoir | isopropanol | 10 (%) |
