1PLQ

CRYSTAL STRUCTURE OF THE EUKARYOTIC DNA POLYMERASE PROCESSIVITY FACTOR PCNA

Experimental procedure

Spacegroup name	P 21 3
Unit cell lengths	121.200, 121.200, 121.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.300
R-factor	0.187
Rwork	0.187
RMSD bond length	0.011
RMSD bond angle	1.750
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.089 *
Total number of observations	300303 *
Number of reflections	26608
Completeness [%]	93.7	92.7 *
Redundancy	11.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		Krishna, T.S.R., (1994) J.Mol.Biol., 241, 265. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	20 (mM)
2	1	1	EDTA	1 (mM)
3	1	1	DTT	1 (mM)
4	1	1		0.1 (M)
5	1	2	protain	20 (mg/ml)