1PLG

EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1991-04-24
Detector	SDMS
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	79.140, 91.210, 141.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.164
Rwork	0.164
RMSD bond length	0.014
RMSD bond angle	0.052 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.054
Total number of observations	87625 *
Number of reflections	10590 *
Completeness [%]	99.8 *
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab	6.7 (mg/ml)
2	1	drop	reservoir solution		0.006 ml
3	1	reservoir	PEG4000	16-18 (%)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir	ammonium sulfate	0.1 (M)
6	1	reservoir		0.01 (M)