1PL3
Cytochrome Domain Of Cellobiose Dehydrogenase, M65H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 65 |
| Unit cell lengths | 139.033, 139.033, 52.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 1.900 |
| R-factor | 0.17433 |
| Rwork | 0.173 |
| R-free | 0.19800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d7c |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.730 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.113 | 0.575 |
| Total number of observations | 256083 * | |
| Number of reflections | 45850 * | |
| <I/σ(I)> | 5 | 1.1 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, 2-METHYL 2,4-PENTANEDIOL, HEPES, CADMIUM CHLORIDE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
| 3 | 1 | reservoir | MPD | 5 (%(v/v)) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 5 | 1 | reservoir | 10 (mM) |
