1PKW
Crystal structure of human glutathione transferase (GST) A1-1 in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.02916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.204, 90.498, 51.334 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 67.420 - 2.000 |
| R-factor | 0.15684 |
| Rwork | 0.151 |
| R-free | 0.20772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.485 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30211 | |
| Completeness [%] | 91.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 4000, Tris HCl, 2-mercaptoethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
