1PKN

STRUCTURE OF RABBIT MUSCLE PYRUVATE KINASE COMPLEXED WITH MN2+, K+, AND PYRUVATE

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	83.600, 109.900, 146.800
Unit cell angles	94.90, 93.60, 112.30

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.191
RMSD bond length	0.011
RMSD bond angle	2.800
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.108 *	0.287 *
Total number of observations	168794 *
Number of reflections	104618 *	9982 *
Completeness [%]	80.0 *	41 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	pyruvate	2.9 (mM)
2	1	1		1.2 (mM)
3	1	1		0.45 (M)
4	1	1	PEG8000	7.9-8.4 (%)
5	1	1	succinate	30 (mM)
6	1	1	pyruvate kinase	6.4-7.0 (mg/ml)
7	1	1	HEPES	4.3 (mM)