1PKJ
Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.984 |
Spacegroup name | P 21 3 |
Unit cell lengths | 110.740, 110.740, 110.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.140 - 2.100 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.23300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 26643 | |
<I/σ(I)> | 26.3 | 7.3 |
Completeness [%] | 99.7 | 99.2 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | nanopure water, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |