1PK0
Crystal Structure of the EF3-CaM complexed with PMEApp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2003-04-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.263, 165.759, 342.409 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 3.300 |
| Rwork | 0.264 |
| R-free | 0.30200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | ef3-cam |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.100 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.081 * | 0.390 |
| Number of reflections | 49594 | |
| <I/σ(I)> | 7.4 | 5.35 |
| Completeness [%] | 99.4 * | 99.8 |
| Redundancy | 7.3 * | 6.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 273 | Yan, S.Z., (2003) J.Biol.Chem., 278, 25990. * |
