1PJX

0.85 ANGSTROM STRUCTURE OF SQUID GANGLION DFPASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-04-01
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	43.114, 81.849, 86.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 0.850
R-factor	0.1206
R-free	0.12840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e1a
RMSD bond length	0.020
RMSD bond angle	0.061
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	0.850
High resolution limit [Å]	0.835	0.835
Rmerge	0.065	0.379
Number of reflections	264548
<I/σ(I)>	7.7	1.8
Completeness [%]	93.8	77.8
Redundancy	3.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5