1PJM

Mouse Importin alpha-bipartite NLS from human retinoblastoma protein Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	79.000, 89.179, 100.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.500
R-factor	0.204
Rwork	0.204
R-free	0.23900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	: 1ial
RMSD bond length	0.006
RMSD bond angle	18.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.073	0.403
Total number of observations	249870 *
Number of reflections	25382
<I/σ(I)>	19	2.8
Completeness [%]	96.0	98.3
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	293	Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.7 (mg/ml)
2	1	reservoir	sodium citrate	0.6 (M)	pH6.0
3	1	reservoir	dithiothreitol	10 (mM)