1PJG
RNA/DNA Hybrid Decamer of CAAAGAAAAG/CTTTTCTTTG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 1999-10-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.701, 41.073, 46.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.150 |
| Rwork | 0.132 |
| R-free | 0.15800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ID AH0001 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.081 | 0.261 |
| Number of reflections | 17368 | |
| <I/σ(I)> | 29.6 | 11.09 |
| Completeness [%] | 96.0 * | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 | 278 | calcium acetate, cacodylate, beta-octylglucoside, spermidine hydrochloride, 2-methyl,2,4-pentanediol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | calcium acetate | ||
| 2 | 1 | 1 | cacodylate | ||
| 3 | 1 | 1 | beta-octylglucoside | ||
| 4 | 1 | 1 | spermidine HCl | ||
| 5 | 1 | 1 | 2-methyl,2,4-pentanediol | ||
| 6 | 1 | 2 | calcium acetate | ||
| 7 | 1 | 2 | 2-methyl,2,4-pentanediol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hybrid duplex | 0.3 (mM) | |
| 2 | 1 | drop | calcium acetate | 12 (mM) | |
| 3 | 1 | drop | spermidine hydrochloride | 0.6 (mM) | pH6.8 |
| 4 | 1 | drop | beta-octyl-glycoside | 0.075 (%) | |
| 5 | 1 | drop | sodium cacodylate | 12 (mM) | pH6.8 |
| 6 | 1 | drop | MPD | 12 (%(v/v)) | |
| 7 | 1 | reservoir | MPD | 40 (%) |
