1PIL

STRUCTURE OF THE ESCHERICHIA COLI SIGNAL TRANSDUCING PROTEIN PII

Experimental procedure

Spacegroup name	P 63
Unit cell lengths	61.600, 61.600, 56.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.700
R-factor	0.245 *
Rwork	0.240
RMSD bond length	0.010
RMSD bond angle	1.492
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.025 *
Number of reflections	6578 *
Completeness [%]	89.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *	Vasudevan, S.G., (1994) FEBS Lett., 337, 255. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	phosphate	1 (M)
3	1	reservoir	phosphate	2 (M)