1PIF
PIG ALPHA-AMYLASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 275 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.700, 114.900, 118.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.300 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.20800 |
| Structure solution method | PATTERSON SEARCH |
| Starting model (for MR) | PIG PANCREAS ALPHA-AMYLASE (REFERENCE 1) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | |
| Number of reflections | 41481 | |
| Completeness [%] | 95.2 | 81.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | cacodylate | 0.01 (M) | |
| 3 | 1 | drop | 0.02 (M) |
