1PI6

YEAST ACTIN INTERACTING PROTEIN 1 (Aip1), ORTHORHOMBIC CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-04-20
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 2
Unit cell lengths	69.120, 154.430, 62.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.500
R-factor	0.219
Rwork	0.219
R-free	0.26300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pgu
RMSD bond length	0.005 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.063 *	0.235 *
Total number of observations	192575 *
Number of reflections	22190 *
Completeness [%]	93.5 *	83.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	15% PEG 4000, 100mM NaCl, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	reservoir		200 (mM)
4	1	reservoir	PEG4000	13 (%(w/v))