1PI1
Crystal structure of a human Mob1 protein; toward understanding Mob-regulated cell cycle pathways.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979, 0.979, 0.964 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.676, 67.676, 95.595 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 * | |
| Total number of observations | 200402 * | |
| Number of reflections | 17488 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 4 * | Sodium Citrate, Bis-Tris Propane, isopropanol, DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 80-90 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 1.0 (M) | |
| 3 | 1 | reservoir | BTP | 100 (mM) | pH6.8 |
| 4 | 1 | reservoir | isopropanol | 5 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
