1PHT

PHOSPHATIDYLINOSITOL 3-KINASE P85-ALPHA SUBUNIT SH3 DOMAIN, RESIDUES 1-85

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995-04-16
Detector	XUONG-HAMLIN MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	47.000, 47.000, 92.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.223
Rwork	0.223
R-free	0.25800
RMSD bond length	0.007
RMSD bond angle	27.020 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.000
Rmerge	0.032 *
Total number of observations	32529 *
Number of reflections	6918
Completeness [%]	91.0 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	sodium phosphate	100 (mM)
4	1	reservoir	sodium citrate	1.4 (M)
5	1	reservoir	sodium azide	0.01 (%)