1PHO

CRYSTAL STRUCTURES EXPLAIN FUNCTIONAL PROPERTIES OF TWO E. COLI PORINS

Experimental procedure

Spacegroup name	P 3 2 1
Unit cell lengths	119.900, 119.900, 51.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.222
Rwork	0.222
RMSD bond length	0.021
RMSD bond angle	3.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.090 *
Number of reflections	8685 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	9.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	0.05 (M)
3	1	reservoir	n-octyl-2-hydroxyethylsulfoxide	0.6 (%(w/w))
4	1	reservoir	octylPOE	0.1 (%)
5	1	reservoir		0.7 (M)
6	1	reservoir	PEG2000	6 (%(w/w))