1PHN

STRUCTURE OF PHYCOCYANIN FROM CYANIDIUM CALDARIUM AT 1.65A RESOLUTION

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1990
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	H 3 2
Unit cell lengths	106.420, 106.420, 176.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.650
R-factor	0.184 *
Rwork	0.183
R-free	0.27200
RMSD bond length	0.009
RMSD bond angle	1.810 *
Data reduction software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000
High resolution limit [Å]	1.650	1.650 *
Rmerge	0.089
Number of reflections	44677
Completeness [%]	90.3 *	65 *
Redundancy	3.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	phosphate	50 (mM)
3	1	drop	ammonium sulfate	0.45 (M)
4	1	drop	sodium azide	0.02 (%)
5	1	reservoir	ammonium sulfate	1.0 (M)