1PHM
PEPTIDYLGLYCINE ALPHA-HYDROXYLATING MONOOXYGENASE (PHM) FROM RAT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.400, 68.660, 81.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 26.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 31034 | |
| <I/σ(I)> | 20.2 | 4.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 25 * | pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cupric sulfate | 0.525 (mM) | |
| 2 | 1 | reservoir | 3.08 (mM) | ||
| 3 | 1 | reservoir | dimethylarsinic acid | 100 (mM) |
