1PGV
Structural Genomics of Caenorhabditis elegans: tropomodulin C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.706, 50.621, 107.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.209 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1io0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 * | 0.243 * |
| Total number of observations | 142014 * | |
| Number of reflections | 16189 * | |
| <I/σ(I)> | 10.6 | 3.5 |
| Completeness [%] | 96.7 * | 85.3 |
| Redundancy | 8.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | Ding, H., (2003) Acta Crystallogr.,Sect.D, 59, 1106. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | sodium azide | 0.01 (%) | pH7.5 |
| 3 | 1 | drop | protein | 13.7 (mg/ml) | |
| 4 | 1 | reservoir | PEG400 | 28.0 (%(v/v)) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
