1PGA
TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.039, 25.084, 51.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.070 |
| R-factor | 0.174 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.970 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.040 * |
| Rmerge | 0.070 * |
| Number of reflections | 2701 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 50 (%) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | NaOAc | 25 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 20 (%) | |
| 5 | 1 | drop | protein | 10 (mg/ml) |
