1PFQ

crystal structure of human apo dipeptidyl peptidase IV / CD26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	71.031, 118.142, 184.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.25614
Rwork	0.254
R-free	0.29800 *
Structure solution method	MIR
RMSD bond length	0.004
RMSD bond angle	0.650 *
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Rmerge	0.126 *	0.648 *
Total number of observations	803624 *
Number of reflections	122631
Completeness [%]	99.7	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	25 (%)
2	1	reservoir		200 (mM)
3	1	reservoir	HEPES	100 (mM)	pH7.5
4	1	drop	Tris	25 (mM)	pH7.5
5	1	drop		100 (mM)
6	1	drop	TCEP	2 (mM)
7	1	drop	glycerol	5 (%)
8	1	drop	protein	20 (mg/ml)