1PFK
CRYSTAL STRUCTURE OF THE COMPLEX OF PHOSPHOFRUCTOKINASE FROM ESCHERICHIA COLI WITH ITS REACTION PRODUCTS
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 112.300, 85.400, 77.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.165 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.030 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 * |
| High resolution limit [Å] | 2.400 * |
| Rmerge | 0.133 * |
| Total number of observations | 93331 * |
| Number of reflections | 27155 * |
| Completeness [%] | 74.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4 (mg/ml) | |
| 2 | 1 | 1 | MPD | 50 (%(v/v)) | |
| 3 | 1 | 1 | fructose 6-pfosphate | 0.2 (mM) | |
| 4 | 1 | 1 | ADP | 7.5 (mM) | |
| 5 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 6 | 1 | 1 | 5 (mM) | ||
| 7 | 1 | 1 | dithiothreitol | 2.4 (mM) | |
| 8 | 1 | 1 | EDTA | 2.4 (mM) |
