1PER

THE COMPLEX BETWEEN PHAGE 434 REPRESSION DNA-BINDING DOMAIN AND OPERATOR SITE OR3: STRUCTURAL DIFFERENCES BETWEEN CONSENSUS AND NON-CONSENSUS HALF-SITES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	150.900, 64.500, 27.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.500
R-factor	0.187
RMSD bond length	0.010 *
RMSD bond angle	1.700 *
Refinement software	CORELS

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.083 *
Total number of observations	20076 *
Number of reflections	8311 *
Completeness [%]	83.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	0.55 (mM)
2	1	reservoir	PEG3000	15-17 (%(w/v))
3	1	reservoir		80 (mM)
4	1	reservoir		100 (mM)
5	1	reservoir	spermine	2 (mM)
6	1	reservoir	MES	100 (mM)	pH5.5