1PDY

X-RAY STRUCTURE AND CATALYTIC MECHANISM OF LOBSTER ENOLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Detector technology	IMAGE PLATE
Collection date	1992-02
Wavelength(s)	0.91
Spacegroup name	P 31 2 1
Unit cell lengths	110.800, 110.800, 73.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.400
R-factor	0.215
Rwork	0.215
RMSD bond length	0.007
RMSD bond angle	23.100 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.077	0.353 *
Total number of observations	78769 *
Number of reflections	19309
Completeness [%]	91.4	46.5 *
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	50 (mM)
2	1	reservoir	ammonium sulfate	2.5-2.7 (M)