1PDO
PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.360, 76.360, 88.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24400 |
| Structure solution method | MIRAS SOFTWARE USED : CCP4 PROGRAM SUITE 1994 STARTING MODEL FOR MOLECULAR REPLACEMENT: NULL |
| RMSD bond length | 0.014 |
| RMSD bond angle | 22.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.272 |
| Number of reflections | 16839 | |
| <I/σ(I)> | 32.4 | 8.1 |
| Completeness [%] | 97.3 | 95.8 |
| Redundancy | 5.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 | pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.3 (mg/ml) | and 9.8 mg/ml |
| 2 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | MES | 0.1 (M) |
