1PDN
CRYSTAL STRUCTURE OF A PAIRED DOMAIN-DNA COMPLEX AT 2.5 ANGSTROMS RESOLUTION REVEALS STRUCTURAL BASIS FOR PAX DEVELOPMENTAL MUTATIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-02-01 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.600, 68.600, 100.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28400 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 28.400 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.075 |
| Total number of observations | 40156 * |
| Number of reflections | 9285 |
| Completeness [%] | 92.5 * |
| Redundancy | 4.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | DTT | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 3 | 1 | 1 | PEG 1000 | ||
| 4 | 1 | 1 | NH4 ACETATE | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | EDTA | ||
| 7 | 1 | 1 | DTT | ||
| 8 | 1 | 2 | WATER | ||
| 9 | 1 | 2 | PEG 1000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | prd paired domain | 0.49 (mM) | |
| 10 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 2 | 1 | drop | DNA complex | 0.62 (mM) | |
| 3 | 1 | drop | ammonium acetate | 0.15-0.2 (M) | |
| 4 | 1 | drop | bis-tris-propane | 10 (mM) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | 0.1 (mM) | ||
| 7 | 1 | drop | dithiothreitol | (mM) | |
| 8 | 1 | drop | PEG1000 | 5 (%) | |
| 9 | 1 | reservoir | PEG1000 | 10 (%) |
