1PDK

PAPD-PAPK CHAPERONE-PILUS SUBUNIT COMPLEX FROM E.COLI P PILUS

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.9879, 0.9792, 0.9788, 0.9667
Spacegroup name	P 21 21 21
Unit cell lengths	62.120, 63.690, 92.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.238 *
Rwork	0.235
R-free	0.27300
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.064 *	0.146
Total number of observations	102568 *
Number of reflections	14203
<I/σ(I)>	15	3
Completeness [%]	91.5	79.4
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG6000	10-15 (%(w/v))
3	1	reservoir	potassium acetate	100 (mM)
4	1	reservoir	sodium acetate	200-400 (mM)