1PDK
PAPD-PAPK CHAPERONE-PILUS SUBUNIT COMPLEX FROM E.COLI P PILUS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9879, 0.9792, 0.9788, 0.9667 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.120, 63.690, 92.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.238 * |
| Rwork | 0.235 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 * | 0.146 |
| Total number of observations | 102568 * | |
| Number of reflections | 14203 | |
| <I/σ(I)> | 15 | 3 |
| Completeness [%] | 91.5 | 79.4 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 10-15 (%(w/v)) | |
| 3 | 1 | reservoir | potassium acetate | 100 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 200-400 (mM) |
