1PCH

STRUCTURAL EVIDENCE FOR THE EVOLUTIONARY DIVERGENCE OF MYCOPLASMA FROM GRAM-POSITIVE BACTERIA: THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-05-17
Detector	SIEMENS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	32.200, 42.200, 52.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.17
Rwork	0.170
RMSD bond length	0.020
RMSD bond angle	25.500 *
Data reduction software	XENGEN
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	1.800
Rmerge	0.045
Total number of observations	18520 *
Number of reflections	6562
Completeness [%]	94.0
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	60 (%sat)
2	1	reservoir	PEG3000	0.1 (%(v/v))
3	1	reservoir	HEPES	100 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	drop	Tris	10 (mM)