1PBP
FINE TUNING OF THE SPECIFICITY OF THE PERIPLASMIC PHOSPHATE TRANSPORT RECEPTOR: SITE-DIRECTED MUTAGENESIS, LIGAND BINDING, AND CRYSTALLOGRAPHIC STUDIES
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.880, 64.570, 124.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.849 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 1.900 * |
| High resolution limit [Å] | 1.720 * |
| Rmerge | 0.045 * |
| Number of reflections | 22300 |
| Completeness [%] | 69.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.25 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 1.5 (mM) | |
| 3 | 1 | drop | PEG6000 | 13.5 (%(w/v)) | |
| 4 | 1 | drop | 37.5 (mM) | ||
| 5 | 1 | drop | potassium acetate | 15 (mM) | |
| 6 | 1 | reservoir | PEG6000 | 18 (%(w/v)) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | potassium phosphate | 2 (mM) | |
| 9 | 1 | reservoir | potassium acetate | 20 (mM) |
