1PBJ
CBS domain protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2003-04-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97954, 0.97940, 0.95667 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.788, 48.586, 56.481 |
| Unit cell angles | 90.00, 107.78, 90.00 |
Refinement procedure
| Resolution | 17.930 - 1.400 |
| R-factor | 0.1924 |
| Rwork | 0.192 |
| R-free | 0.20200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.930 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 27732 |
| Completeness [%] | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG 400, Hepes, MgCl2, NaCl, Imidazole, Glycerol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
