1PB7
CRYSTAL STRUCTURE OF THE NR1 LIGAND BINDING CORE IN COMPLEX WITH GLYCINE AT 1.35 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-04-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.660, 73.020, 96.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 1.350 |
| R-factor | 0.1941 |
| Rwork | 0.194 |
| R-free | 0.21600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.280 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.065 * | 0.280 * |
| Total number of observations | 383080 * | |
| Number of reflections | 57637 | |
| Completeness [%] | 87.7 | 41.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | PEG 1000, sodium cacodylate, lithium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.0 |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | PEG1000 | 20 (%) |
