1PAM
CYCLODEXTRIN GLUCANOTRANSFERASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.930, 74.450, 79.120 |
| Unit cell angles | 85.20, 105.00, 101.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | B. STEAROTHERMOPHILUS CGTASE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.100 * |
| Data reduction software | MADNES |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.600 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.080 |
| Number of reflections | 111795 |
| Completeness [%] | 86.1 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 1% PROTEIN, 20% PEG3000, 20% 2 PROPANOL,0.1M NA CITRATE, PH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG3000 | 20 (%(w/v)) | |
| 2 | 1 | 1 | 2-propanol solution | 20 (%(v/v)) | |
| 3 | 1 | 1 | sodium citrate | 0.1 (M) |
