1P9W
Crystal Structure of Vibrio cholerae putative NTPase EpsE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.412, 104.412, 166.918 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.700 |
| Rwork | 0.238 |
| R-free | 0.28400 * |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.348 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE (V. 2.02) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.152 * | 0.577 * |
| Total number of observations | 160321 * | |
| Number of reflections | 15414 | |
| <I/σ(I)> | 11.1 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.40 * | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 * | 287 | PEG 200, 3-morpholinopropanesulfonate, AMP-PNP, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | TEA | 0.1 (M) | |
| 3 | 1 | drop | 0.5 (M) | ||
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | TCEP | 1 (mM) | |
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | reservoir | PEG5000 MME | 12-18 (%(v/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 0.15-0.20 (M) | |
| 9 | 1 | reservoir | MES | 0.1 (M) | pH6.3 |
