1P9Q
Structure of a hypothetical protein AF0491 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-17 |
| Detector | SBC-2 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.875, 44.254, 61.418 |
| Unit cell angles | 92.74, 118.02, 109.67 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.219 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (+ PHASES + SHARP) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 35721 | |
| <I/σ(I)> | 23.2 | 3.8 |
| Completeness [%] | 92.2 | 84.6 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 294 | Sodium acetate, PEG 3350, Ethylene Glycole, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K, pH 7.80 |
