1P9E
Crystal Structure Analysis of Methyl Parathion Hydrolase from Pseudomonas sp WBC-3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-29 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.2828, 1.2832, 0.9000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.020, 85.020, 199.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.218 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.673 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 180629 | |
| Completeness [%] | 95.9 | 96 |
| Redundancy | 5.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | PEG400, Na-Acetate, CdCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
