1P8Z

Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0052
Spacegroup name	P 31 1 2
Unit cell lengths	114.220, 114.220, 93.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.208
Rwork	0.208
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.580
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.077 *	0.267 *
Number of reflections	21012	2115 *
Completeness [%]	97.4	99.2
Redundancy	3.6 *	3.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	20 *	0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG400	12.5 (%(v/v))
3	1	reservoir		0.1 (M)	pH5.0
4	1	reservoir		10 (mM)