1P8L
New Crystal Structure of Chlorella Virus DNA Ligase-Adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.059, 60.574, 70.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.950 |
| R-factor | 0.26641 |
| Rwork | 0.263 |
| R-free | 0.30350 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.575 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.06) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | |
| High resolution limit [Å] | 2.950 | |
| Rmerge | 0.092 | 0.640 * |
| Number of reflections | 8265 | |
| Completeness [%] | 96.9 | 95.3 * |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | 4-6% PEG 4000, 50 mM HEPES, 50 mM sodium acetate, pH 7.5, hanging drops, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | 0.1 (M) | ||
| 5 | 1 | reservoir | PEG4000 | 4-6 (%) | |
| 6 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
| 7 | 1 | reservoir | sodium acetate | 50 (mM) |
