1P8K
The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.462, 72.841, 65.231 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.247 |
| Rwork | 0.239 |
| R-free | 0.26200 * |
| Structure solution method | SAD |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.933 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.040 | 0.210 |
| Total number of observations | 58621 * | |
| Number of reflections | 16749 * | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 90.4 | 66.2 * |
| Redundancy | 12.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 22 * | PEG3350, KCl, MgCl2, sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350, | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | sodium citrate | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.0 |
| 5 | 1 | reservoir | PEG3350 | 16-22 (%) |
