1P8I
F219L BACTERIORHODOPSIN MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-04 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.930 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.074, 61.074, 110.228 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.860 |
| R-factor | 0.1914 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m0l |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.084 | 0.759 |
| Total number of observations | 492763 * | |
| Number of reflections | 21424 | |
| <I/σ(I)> | 28.7 | 3.4 |
| Completeness [%] | 99.5 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 295 | Gabriele, R., (1998) J. Struct. Biol., 121, 82. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3.3 (mg/ml) | |
| 2 | 1 | 1 | sodium potassium Pi | 1 (M) | |
| 3 | 1 | 1 | MPD | 2.5 (%) |
