1P8I

F219L BACTERIORHODOPSIN MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-04
Detector	ADSC QUANTUM
Wavelength(s)	0.930
Spacegroup name	P 63
Unit cell lengths	61.074, 61.074, 110.228
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.860
R-factor	0.1914
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m0l
RMSD bond length	0.022
RMSD bond angle	0.041
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.930
High resolution limit [Å]	1.860	1.860
Rmerge	0.084	0.759
Total number of observations	492763 *
Number of reflections	21424
<I/σ(I)>	28.7	3.4
Completeness [%]	99.5	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	295	Gabriele, R., (1998) J. Struct. Biol., 121, 82. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3.3 (mg/ml)
2	1	1	sodium potassium Pi	1 (M)
3	1	1	MPD	2.5 (%)