1P7H
Structure of NFAT1 bound as a dimer to the HIV-1 LTR kB element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Wavelength(s) | 0.900 |
Spacegroup name | P 1 |
Unit cell lengths | 74.107, 80.321, 80.308 |
Unit cell angles | 71.20, 78.97, 78.94 |
Refinement procedure
Resolution | 30.000 * - 2.600 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.25400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.380 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | |
High resolution limit [Å] | 2.600 * | 2.600 * |
Rmerge | 0.060 * | |
Number of reflections | 51229 | |
Completeness [%] | 93.9 * | 58.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 18 * | pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
10 | 1 | reservoir | spermine | 2 (mM) | |
11 | 1 | reservoir | dithiothreitol | 2 (mM) | |
12 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
2 | 1 | drop | spermine | 2 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | glycerol | 8 (%(v/v)) | |
6 | 1 | drop | 350 (mM) | ||
7 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.1 |
8 | 1 | reservoir | 100 (mM) | ||
9 | 1 | reservoir | PEG3000 | 14 (%(w/v)) |