1P7B

Crystal structure of an inward rectifier potassium channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	I 2 2 2
Unit cell lengths	92.840, 105.620, 258.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 3.650
R-factor	0.295 *
Rwork	0.295
R-free	0.32900
Structure solution method	MIR
RMSD bond length	0.017 *
RMSD bond angle	2.000 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.850
High resolution limit [Å]	3.650	3.650
Rmerge	0.087	0.412
Total number of observations	87944 *
Number of reflections	14455
<I/σ(I)>	4.9	1.8
Completeness [%]	100.0	99.4
Redundancy		6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	20 *	PEG-400, Magnesium Acetate, Glycine, Hega-10, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)	pH8.0
2	1	drop		50 (mM)
3	1	drop	Tris-DM	0.05 (mM)
4	1	drop	protein	9-12 (mg/ml)
5	1	reservoir	PEG400	20-22 (%)
6	1	reservoir	magnesium acetate	50-100 (mM)	or MgCl2
7	1	reservoir	glycine	0.1 (M)	pH9.0