1P7B
Crystal structure of an inward rectifier potassium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.840, 105.620, 258.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.500 - 3.650 |
| R-factor | 0.295 * |
| Rwork | 0.295 |
| R-free | 0.32900 |
| Structure solution method | MIR |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.850 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.087 | 0.412 |
| Total number of observations | 87944 * | |
| Number of reflections | 14455 | |
| <I/σ(I)> | 4.9 | 1.8 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | PEG-400, Magnesium Acetate, Glycine, Hega-10, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | Tris-DM | 0.05 (mM) | |
| 4 | 1 | drop | protein | 9-12 (mg/ml) | |
| 5 | 1 | reservoir | PEG400 | 20-22 (%) | |
| 6 | 1 | reservoir | magnesium acetate | 50-100 (mM) | or MgCl2 |
| 7 | 1 | reservoir | glycine | 0.1 (M) | pH9.0 |
