1P6P
Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-02 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 48.140, 48.140, 135.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22 |
| Rwork | 0.212 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | axolotl liver basic FABP model (unpublished) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 17.582 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 * | 0.252 * |
| Total number of observations | 47279 * | |
| Number of reflections | 5917 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | Di Pietro, S.M., (2001) Acta Crystallogr.,Sect.D, 57, 1903. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27-30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | Tris-HCl | 0.05 (M) | pH7.4 |
| 4 | 1 | reservoir | PEG1500 | 30 (%(w/v)) |
