1P6F

Structure of the human natural cytotoxicity receptor NKp46

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Detector technology	CCD
Collection date	2002-12-06
Detector	MARRESEARCH
Spacegroup name	P 61
Unit cell lengths	85.731, 85.731, 59.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.200
R-factor	0.207
Rwork	0.207
R-free	0.25000 *
Structure solution method	MIRAS
RMSD bond length	0.008 *
RMSD bond angle	1.470 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.460	2.340
High resolution limit [Å]	2.200	2.200
Number of reflections	12472
Completeness [%]	97.9	87.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	18 *	PEG 2000, MOPS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	PEG2000	8-11 (%)
3	1	reservoir	MOPS	0.1 (M)	pH7.2