1P5U
X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 178.816, 69.620, 45.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000* |
| R-factor | 0.1682 |
| Rwork | 0.166 |
| R-free | 0.22500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Refined model of binary Caf1M:Caf1 pre-assembly complex |
| RMSD bond length | 0.025 * |
| RMSD bond angle | 2.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 2.150 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.086 | 0.240 |
| Number of reflections | 34536 | |
| <I/σ(I)> | 5.4 | 3 |
| Completeness [%] | 88.6 * | 82 |
| Redundancy | 5.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 8000, calcium acetate, cacodylate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 55 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 16-17 (%) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 4 | 1 | reservoir | calcium acetate | 0.2 (M) | pH5.9 |
