1P5T
Crystal Structure of Dok1 PTB Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9798, 0.9800, 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.110, 56.250, 99.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.500* |
| R-factor | 0.2226 |
| Rwork | 0.218 |
| R-free | 0.26500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.780 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 14730 | |
| Completeness [%] | 79.2 | 39.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 16 * | PEG6000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | MES | 10 (mM) | pH6.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 28 (%(v/v)) | |
| 6 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
| 7 | 1 | reservoir | dithiothreitol | 10 (mM) |
