1P5S
STRUCTURE AND FUNCTION OF THE CALPONIN-HOMOLOGY DOMAIN OF AN IQGAP PROTEIN FROM SCHIZOSACCHAROMYCES POMBE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.653 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.884, 68.666, 35.309 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 21.000 - 2.220 |
| R-factor | 0.19921 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | Sulfur SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.107 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.600 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 6890 | |
| Completeness [%] | 97.4 | 81.5 |
| Redundancy | 16.45 | 8.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
